中文摘要：

開發(fā)有效的金黃色葡萄球菌（S. aureus）疫苗一直是一項具有挑戰(zhàn)性的工作，過去多年眾多失敗的臨床試驗也證明了這一點。在本研究中，我們配制了一種包含高度保守的“兼職"蛋白——丙酮酸脫氫酶復(fù)合物E2亞基（PDHC）的疫苗，并證明該疫苗在多種小鼠疾病模型中能夠誘導(dǎo)針對流行病學(xué)相關(guān)葡萄球菌菌株的保護性免疫。雖然抗體應(yīng)答有助于控制細菌，但在血流感染模型中，抗體對于保護性免疫并非必需。相反，疫苗誘導(dǎo)的全身性免疫依賴于γδ T細胞。此前有研究認為，先前接觸過金黃色葡萄球菌可能會降低疫苗效力。然而，PDHC誘導(dǎo)的保護性免疫在先前接觸過金黃色葡萄球菌的小鼠中仍能促進細菌清除。綜上所述，我們的研究結(jié)果表明，PDHC是一種有前景的、不依賴血清型的候選疫苗，對甲氧西林敏感和耐甲氧西林的金黃色葡萄球菌分離株均有效。

英文摘要：

Developing an effective Staphylococcus aureus (S. aureus) vaccine has been a challenging endeavor, as demonstrated by numerous failed clinical trials over the years. In this study, we formulated a vaccine containing a highly conserved moonlighting protein, the pyruvate dehydrogenase complex E2 subunit (PDHC), and showed that it induced strong protective immunity against epidemiologically relevant staphylococcal strains in various murine disease models. While antibody responses contributed to bacterial control, they were not essential for protective immunity in the bloodstream infection model. Conversely, vaccine-induced systemic immunity relied on γδ T cells. It has been suggested that prior S. aureus exposure may contribute to the reduction of vaccine efficacy. However, PDHC-induced protective immunity still facilitated bacterial clearance in mice previously exposed to S. aureus. Collectively, our findings indicate that PDHC is a promising serotype-independent vaccine candidate effective against both methicillin-sensitive and methicillin-resistant S. aureus isolates.

論文信息：

論文題目：Conserved moonlighting protein pyruvate dehydrogenase induces robust protection against Staphylococcus aureus infection

期刊名稱：PNAS

時間期卷：21 (36) e2321939121

在線時間：2024年8月26日

DOI: 10.1073/pnas.2321939121

產(chǎn)品信息：

貨號：CP-005-005

規(guī)格：5ml+5ml

品牌：Liposoma

產(chǎn)地：荷蘭

名稱：Clodronate Liposomes&Control Liposomes

辦事處：靶點科技

Clodronate Liposomes氯膦酸鹽脂質(zhì)體清除小鼠金黃色葡萄球菌感染模型里巨噬細胞。荷蘭Liposoma巨噬細胞清除劑ClodronateLiposomes見刊于PNAS：保守型兼職蛋白丙酮酸脫氫酶誘導(dǎo)針對金黃色葡萄球菌感染的強效保護。

Liposoma巨噬細胞清除劑Clodronate Liposomes氯膦酸二鈉脂質(zhì)體清除巨噬細胞的材料和方法：

In vivo macrophage depletion

For macrophage depletion, mice were injected intravenously with 100 µL of clodronate liposome (Liposoma) suspension/10 grams of mouse weight 2 d before S. aureus challenge. The same amount of control liposome was administered to the control group.

巨噬細胞清除材料和方法文獻截圖：